	   SnapGene      \ATCGATAGGTACCGAGCTCTTACGCGTGCTAGCGAAACTGAAACTGAAACTGAAACTGAAACTGAAACTGAAACTGAAACTGAAACTGAAACTAGATCTGGGTATATAATGGAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCACTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGTCTAGAGTCGGGGCGGCCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCCCAAGCTACCATGATAAGTAAGTAATATTAAGGTACGGGAGGTACTTGGAGCGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAACGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCT  $¹   
      	      	                     	                  	                                       	   	               
                              	   
         	               	      	                                          
            	                              ;      #               
      #                        
               
                                                         	      	                                    	            
      	                  
                                                      $                     
                                                         
         	                                                       
                  	               
                        
                                                
                 
 f$%ŸP@R Å9&/QN)ù˜	Ðá]«	 c ï ªS–P Õ g™ –ýY	AØcbo	 ™ « ê '0úŒ¬¶ n &©Æ6m—\�O„U	™	2¹Ñá´^ :JcR4ä*EQ+â +˜×é¢ É ð ö Vé*–ÜØ~'=·Þ	º
«—œQjH  d ~ ‡ 
U"7qÇác�±ñ	¦¯ ž3?º
;
z
TŸWwúå	 „1�j	H
Ë Ähš4šÛúW$
â
ø
BÙ…“c v Ö ~ZE¡âùŸ¬Ö	€
½4 ¨—Kþ3	ö	÷N2 9Êkoq|Ž@
	Z	e
dòp» í (1Öªû­	 š ë y7#	Ó
a ¬ Ž'a’Ö0‹ƒ¡	
½
é
�  x{i¹^	®'· o œ ó ›Úæ‘˜P8UtífV	e	š	[
º_ ! ' - 3 9 ? E K Q W 'yª ÿ±Å]Þ…Ü3EâBh À ü7in¨JÕ
Òê7ñ Ÿ ®ÇNa‚£XµO Æ Ì ù ¿”ÇK�Îp©
mT  iì‚‡^Só5ëô	,p“ö ;Ñ+9:	%KØãì
 ^ qdšåðøFR»
"
P
Rc‡f Â¶Évt† Ð,^†Ü}™Øåæ’Ü	 ƒ¦»ÿêëÛ¶
£	 ©l$µ%	å
®< è -S—ÙU;>¸h	Ü	š�a  Ê ñ ÷ ê¯ÃÌg¨´
»
¬¬Rï W+›æß	l
Ú
˜Þàí  �ÕÝ�(	/
&{m+k=CI
 Ôn�!‘²è§	€^ ˆ ” -àBV8‡Èâ…£	^
¥
p˜œ e O#r]d{ò	Ü
;   ¤¿
§°ú „ ® ½ ”�Ô’	Ä	Ç	,
T
b
ë
^ç‰Zâ Ï Ÿõvû@{$–²	
<
n
{
 û â)”4�ý»Ãi¹	í	¤xæ 2Y�zˆ¬2Éª	
9iUð X•ª ci­…ãê	 ä DÝ‰Ò2á	W
™
Ë
û
	 \¿ÑŽÀ‡	 Ì
 ± Ðê<Øk	I
�6 i� 5%µ9
à
)ƒ‘”m¹  ÅÜ¹!	
ã
CÚd	  ›’›ûSù
¢ª ›æX¼j×`	½	Zy†�¥¿ÇR [¦z¬ãYúýËÎÙb	i
�
§E ü ð>Íkv Ç	C	p	¿	1
°¾ÈT¿*? w F¢»×	‰
�ˆ	 × ³Ÿ­„
}\5 ×36í—k“˜¹q®	[O3	  Ñ*L�	Ð	²y‹ r `sº×ð-ZŸÿ÷	¬
Öø-4  �èø	)©ÌeŒl”,	4	æ	
@
î
(Í lp°ß�*R	[	•	f
Åôé¼ � :’r}ó wN	®	Ê	ž
o»ëe?F�K }@¾A×|	óq —Ë÷s®±	ô
{ÁÉk… î #O(üX	Ï×QOàa ˜ ¨bÖ[€èãDP¸  õ �è	)2Û×pƒ¥®G 3p{�§ù#«Õ	
¼M1 ì �`zÕ/Š 	 › ž ­ûh§I`TWÛìd	Z
Ô
AN ] Å hp¾“†òÐï8Qê9	¨
b
 Ï]…¨Û|å$	s ç �®:§	g	k
Ù
%l*`Ÿ<B “ A\z„¢	¦	]
¾
—›ù ­ áu(“úy1zŒh•Ó©	±	¸	
a
ê
h	 Ü[bˆÑ×†	Ê
Ž  šåR´¸_	8
¡(‚Ž©¾Æ¸ ¥y=juºüÊÍB	ƒ
Ž¯)  q 2ìŒ(
d’“œ	 
?
Š Œ |°ÿvM	Q	„ — ô œça2ozŒâ'CW	
O»ÎÖß`¤ � gŒ¬½Îg’…_ñäV‰ëî§
 Ûàtù@Ð9gyf	¥	\
`
É
$k–šA p ‹ ™ë<	Q‘uÉ›	7
�‰ Š – «¼fs ;1^ny‹ðˆáBx6
#Íj; (e¶²³´µDXmx‰ŠÂÃ†‡ÝÞßà@A`a3
4
5
"4F²³ÀÊËÌCDiBCDþÿ )Ä, Û 1€·W¶ÆOYÄ[bnÁãEM*Å-# " ( . 4 : @ F L R X )z!aœõEß¢¦‡H	5G´IE¯ $ k Ò ]Lj©·Pcœ	Âä8 ø8¸Xò:E	µ	Ö
þ
ÓëFNœ+žÁÆ. ¶ Á Ü 2�ÀÇ\Ž	�
 : ýoòZKÅos£ò
 {	VÁ	
61 þ ="b‹³/öþb	Y£µJ¶¹&ÿ!P# # ) / 5 ; A G M S Y   	*¯È=Cy¡O£ÏÊEˆîr	HƒF°V% ß�âFƒƒà¤Ü§I	Å
rÀ l Ç Ó 7TXk•ÈCHô	x
~
U w�ÀL€�¤¹5d�	 
%‘ Í ¢MQe*
nå9 ú ^•ª¸ÏqË�œ
ª
Þ
Ã  ùY¥bÑ¶	×
Oq�÷/ i Aƒéó_TîìF	‹
ÔGÝ”Â
 g u³}1,ŸÇ jí9Q¹Sˆ;ô6õ	ÿ
	ìò- 3ŽÁŒÔ�	Í	/÷ Â ‚­ã<æÒ:]‹	ŠL.|¡Õ Ý µ¿ÈÜ_,Ä;	3=`&Ùäí!;ÏS
 y · %ü	Ž
Î
ÐBI   þ[ªÆÎðÙ	Q
hgó rUp°È%L½=p¤?	
q
, _ ótfæùGt³¼	Sˆ‘ÆÉÚ eCÃ›ñSÍx	‚	#
¡
d |Ã=I÷ß2u© 
·¡éò
 Ó"Â	7î\‡“  WÊiÕ*	ä	w£2 ¦0ÙGæ
-Z“Ó	¶ Ù Ì/;J_c‰´ÝðUš¡÷ÿMÀ¤K·'8" ÿ #SŒA~Šc
/^Ø Q >Ñ-H‡î?çOÝµº $ * 0 6 < B H N T � ¨ #¼üö†
–
·
„±W Z  +G°É>¢P¤§ëËFQïü& ¡ 
@JP„zµì‰s	˜·G² D»Ð -Üt
Á
Iwîô¤¼ 
 m„1¶«	0	æ
X¯ � ªØàžãö¨™	J	Æ
žŽ	 .æG8~M¯r7 lï„á%¥Ý&	s=Á	 b Ô 8.˜•©b\ m ª é %*5l–I—1‹¡³µ † È UT<b�°¹0Œi	Ý	ð	y
¹
›žVùä u 8YÃÉD™ÚVø?		õ	d

 &‘xhÕ6‚©¼
Ò
`­& & , 2 8 > D J P V ¿ ò xÙå�M°Ä7seé
6ð Ë ø ë�ŽÍ™÷	!
†JSo×	’ ‚Á¥ºþµ
‘Úµ
u­… ÂË,Rfšœ.
³
™Ýßìî £N,ßm€ çÛ
o: ƒ ¼ Î 1XôÂ‘	Æ	+
m
]æ©Yá ° ã CÏé¾;¿à	V
˜
5	 »i¼	ß
Ql û «ï
ÌžÆ	o	0
ˆ
|>D  _Ö5¹ÐÖ–)pž	Ï	«
í
', ‘–örÞ)Ö”	É	�
ó
Änºè>J
  Õ¦o‚"PN0 \ Ä ú„_¦SPéŸ	@r	 ZÒ¨	·	Ø
)_žø  ¤c’³>
�Å( „ê`ã&¤	_
¦
ÕŒÞ•™£ ‰ • ô:CWˆïZ_mw†G	!H
ýÃ j Ñ ÷9¢�	´	Œ
›� Bx.áýUÉDíq%0þ
 Sv¡¸€ó	}
Ý
 f h ²@PRèí|~Ü ´¾ÓÛ^ÃŠ	2<Ïö-{ Î ¯©Ç$BsÂÍïe<²>	w	p
ÅÈ  <HÔñö ¢1~¨ ¥Ë:R>T‰L,NÒ	
.´7× € "Iº•
À
—íó±û 	 -kî×åõ07}˜		
W� t … )4Â–aŽ/‹ï	c
0Šø[´ã ¾ ä�%�wÔ6˜ö_è	5I„Ö ¯ » Bó+ÁÊÞÁ�	Å	-
U
¨ë Õ	•ÅÕ“	»	È	Î	ì
ò
¹ç= Ã ƒ=
?(M Ð öwü9%Y^l¡Œ	³	 ‹šü$/¢ R ®½Úç·ÆAÁî;=	v	o
|
ÛÍ  Öä=/|Ó—	
”
V–¡06}Ö ã*•ÀÉÝ`ÀÄõ.º	î	4ƒÕæ<ê Õ¼Ù8ÅXk<	u	•>'/™Ú#Ì$ £ Þ b†‡¶·Ô567žŸ¼½¾¿ü-Wj®
”üýyzÔå"Ðçèé ¤ ß Lcˆ¸û þC\ýÅ¯
š¥þaÒØåú{îÑT üuK{‰�Æ«	E
=jÓñ|ïCv ¥ MZd‰¹|ž €­JÊù	ý	‹›ÿYJÒ ¸ à &"ÂD]3þ�
°
Ï
:¦Ùæ–ÑU z 3’ìò¡ÿ 
øVbÜáû!« dL|‚F
>Éðh
  ÿýv\jÇk:ô «Ò†ÏÇäÚ	è	ë	R
y ®öŠñ¬	Ôéòi}Dw å !Š&MÓw®¥¶@	þ	X
ŒK [eŠºÞq}Ÿ±�/Îé>q
Ã
ü
M ¾ sVÉ`3Ëú	v
š
Ì
*-ð#Ó ¦ ENFú+¾g K.	â	r
œZ ` á ôgn�½	±
Ð
§Û ]JÿuÁˆ	Í  ¹ '#ÀÃêÒ4–úH’
ð
;T‰’öÚ—¦ÒtÊ E^uç´�
K!çÇV { f“í«!=Äœy	
M
¢
W" 4½ëD¢ŽÙÎ6		ƒ	e�¬
 Ñ�óòTl	B
J
ùÏâ ²  GÞ	
$
cÝü7 ÄežMø—	?•nª 3ƒT	à* } •!6>}Æà&¶
:
G
vÊi }jnáJž2	á
÷
„’ö3ñº
   w¸"	]	
è
   šþÝ¢Dg  Æ]oºó
O
l$ëeõ	 kÈä#´êä
Û;  ÔTåÛ	z« Ó†¤
] œ“œü‡«#ÈÃ	ì	S
8£ïˆ” h¬Ðâé	ú
   œ‘Ö 	Yx�¤ ²XØa	¾	h
Ç[‡¦S¾ rçï÷Ë,Yj‹¸k­+	å	Z±ÌÕ3x ¯­?½ò{	­	zÀÈêój~E€ §"ÚNZçøÔ	·LF o\§Ú{HÔ.ÏÚ8	c	Y
M ’ æ ­‡x[y”¦	…	°	j
g; 1‹ä't¯ûþÌ·A	L	P	ÿ	‚
� ¨½ƒ f‹Íßø?Ï�Èê
È
Nˆ@  Ú »0`dr~µV›N0lwÁ¡¥?2
±¿ÉLA®(#+ µ \K¿ññr›	D	ý
9À ý <<Š ²Þ¢ ‚ÎÛq	À	
Ä
‚¸¿U 6€”WBG£Êd4
)
w
›
$�
  t¤aÐŠ
ñ0 x $�¬â‹åÌ	û	Í
._‰AHÔR T¼Ì	�	Ø	 
+�gÙ Òh è!)	ã	Þí[’ Ø .GIˆíï@ F¿] § ?FO´û,õPs
…
v¶»	 � ªL®/	~�q6 a © $7˜”÷¯	¸
¨² % + 1 7 = C I O U Øý´r	�ÙÑ
…tn ‚ â 0Îè½:l™¾—
²
\~XÜ4 õ4î(ºho	n	‡
P
  [ £^¥ROž	Ä' Cv	ý ±?QÒ¨ìÌ±Â‰	ÄÎõz !,HÊK+GM ðÑ	³Œ s º Aò($vÓ5—Šñ
¸H§³  Q¶.Àí  	“
U5 ¢ Ka…Óú»ØÄB[ôûVt	­
“™ûÑ×äù.‚˜ÓË {!t‘ÁëñJ€Iø	D
<Š÷Ûàu
 Ñõ
ç	xè9È Eù*.Í_fvèµ-	u
Â
)Lï"½  Iém•‘
ï
Jõ¥sW ¼ŽªF�Ý5	~	
A
L
Î	 | m”à2Åß�1	S	–	
ö
õ  ™nã"³\	N
ç
f#ê g…ªá£
ÿ q±î�¬+>·¬	z	g
YÆX°Ë½ ‘ nÙ†s~÷x	7	K	O	„	¯	fŸ¼‚:L ´ ;¾�Úš
Ç
�@­‡ 5“«áä£Ï	€	Ë	(
Ÿ
f@G� Ž ìŸEô©(	upì‘ /Íç³'9Øm	s}m BN°ÃÃô ñÒù u~�AI¿óUC
úÐßã� Hô}	K
8r
 ˜â©²Þàõ
eÊþ ³ m…	'	

'
t€?†9 ðÌøt²&H|ÂÞl€Â ¯°±¦§¨ß	%
&
dý>8  %„  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                                               	                                                                                                                                                                                                                                                 	                        
      	                                                                  
               2   1                     1   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                         	                                                                                         	        
  	                      
     2 1       1 
  QÇ  R3  R9  RE  T_  V¡  Y»É†[�s…+B|ÂÛŒ1þ3ì›ä
¢xµR	Fr¦^^Œß ’ÎR	†ˆ²ÍëFd*Û%	ÛÛØ	w
ÞÙ~a�¹k–EèM
íF‡çÛOi¼	QE ¾
ù Ü¬4HË'·Á	68jnd
ºÛ ÆØrŠÒø
å Ñc �&{�{‹	ü Õ �{8c   [óx¿4+…	9l+Ñ	~åW¾Æ]  cÜ�¨¬
	3	
T4H© 5	$vÓÍèLO
e#	Ò[5¨w { “1Ä
	Ì	lß0	ôjnº6“Cjnk´O
ä
eº5 Õ �&{£3#cO5éõs  lßr—ÇN	»Éôm�5vûc] cäïEQQbÅg¬i
…
8Xk«=y	 © Ør5	ŠûÌ4otvûip Ovêûi�…{X� eŠ#]LÒ4–é36	#¿ !Ù‰lßô1·¬Y[	1i$vÍÓèL+žlå
~F
É©£¾
ùáy
žù]cäEQdšðR"
c~÷ #	3íã¹úýË	�
§OÊ
	Y	j	H
coÞ–	õ
É ð ö éº
«QK.	œj	H
ãúýË�
§3í¹	O	€	Ÿ
fÆ ”ÇT|I23ipí°ã�*¹úýË	f
�
à
Åƒ‘§O¹¼~÷Þ–	õ
»×	�ù Îp©
¨ #ö·
±‘ †x	„	¯	f:JÌ�	 
vµÑ
± 6� ¼=„Z“–
uWvµÑ
æ ­¦	j
;§ ûõ…
Évù
Ê�”münaÿJÖÛ�£ñh¬Èâé	Ó!‚�M
X
Ð
" ~�IC
ßó ›æV	º_D-àÈ{–á	
´
p ¬œ ‘UíÀêH;‰öÚtaÖ�ÿÛ£Dá	´
¬ünJñip°�*f
à
Åƒ‘¹¼§ æ ­ûõ¦	j
…
;wLdÊù	Òßf¢
 œ‘Ö 	Yx�¤q±î�¬+>·¬	z	g
YÆX°Ë½i� 5%µ9
à
)ƒ‘”m¹  ¤¿
§°ú q 2ìŒ(
d’“œ	 
?
Š›æX¼j×`	½	Zy†�¥¿ÇR [ £^¥ROž	Ä'^ qdšåðøFR»
"
P
Rc‡f9Êkoq|Ž@
	Z	e
dòp»| m”à2Åß�1	S	–	
ö
õ6€”WBG£Êd4
)
w
›
$� q Œ 9Ê2koì|Œ(q|Ž¨
d’—°ÿ@Kv“þ
	3	M	Q	Z	œ	ö	 
?
e
÷dòNp„Š»2f h  6Stv€”¡²@PWBGRè£¸Êd¤ía4|~€Ðó	
)
w
}
Š
›
Ý
ñ$0Ü�Ó"Â	7î\‡“û «ï
ÌžÆ	o	0
ˆ
|>D¶ Á Ü 2�ÀÇ\Ž	�
 :Hô}	K
8rí (1Öªû­Ð öwü9%Y^l¡Œ	³	 ‹šü$/¢  q õ .�Óè	æ2ì)2GŒÛ(×
8dpƒ’~“"M¯œ	Â	 
?
7rî7\‡“Š¥®G ÿýv\jÇk:ô  î     é  [         [ld'ÄîœÈäÅxW…xW…xW…xW…xW‘¸	™`S Úðž(%O}%A^«x‹õ®Š‘‚¥€ÝZ®- DP†5ÙÁW§úV¦£yàUçâ½ƒ×z-ç«z`ŠÖ:­FFÙ…pØz«Q#ØüP¦'AQyš«TaÀ^uÈáKR¡BþŠYÄ¥Pš¥EÚèÕ Y×™{wX™Û`ÑÕÕE�mR—­V‘Ýv…Ê×“GÚmuYïZ`þ/ª}}§â÷u¸þ¬To×DGÝ‚úèJ‰tã€iÝƒ`ay�Y.D°<<ëGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*©SRUû�X‚U±”Ëþ„O­KNúð€ÌaæË<Þ˜xºêm�{$ðÓ;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u¨ö³ÚÐã5â€P1°ŒºÎiÏOÔçØe%%šPœ' L<ÖU›©ªÚvâ"‚ªˆ…±’yvs»ÛUÕq]WW“U�âÿHÔ�ô]«.  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="9"><Feature recentID="0" name="GFP" directionality="1" translationMW="26797.80" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="148-861" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Aequoria victoria&lt;/i&gt; green fluorescent protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="142-147" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="7" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3778-3826" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3840-3931" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3061-3165" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="901-1022" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3192-3647" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1441-2029" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2991" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2200-2991" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2992-3060" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>43cca133-d86c-4ee8-8238-6926f7159c2a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:52:57">2019.8.6</Created>
<LastModified UTC="8:19:47">2025.12.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="147..860" colors="green"/></TopStrand><BottomStrand><ColorRange range="147..860" colors="green"/></BottomStrand></StrandColors>  î     é  \         \ld'ÄîœÈäÅxW…xW…xW…xW…xW‘¸	™`S Úðž(%O}%A^«x‹õ®Š‘‚¥€ÝZ®- DP†5ÙÁW§úV¦£yàUçâ½ƒ×z-ç«z`ŠÖ:­FFÙ…pØz«Q#ØüP¦'AQyš«TaÀ^uÈáKR¡BþŠYÄ¥Pš¥EÚèÕ Y×™{wX™Û`ÑÕÕE�mR—­V‘Ýv…Ê×“GÚmuYïZ`þ/ª}}§â÷u¸þ¬To×DGÝ‚úèJ‰tã€iÝƒ`ay�Y+‘,:ÄÑØeÚJ�Õ÷^E�!UXêj!Zˆcš:š‰}¦S�eu)u×Z6¶©Š´+@"
ªTÔ•~çV •leòÇÆ?¡ë_ÒÓ¾¼ 3 Øy²Ï7¦.º›c^ÉB<4ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�j=¬ö´8Íx l#î³šsÓõ9öIIf”'	è5•fêj¶�¸ˆ ª¢!ldž]œîöÕu\WUÕäÕd8¿Ò5#B=jî  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="9"><Feature recentID="0" name="GFP" directionality="1" translationMW="26797.80" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="148-861" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Aequoria victoria&lt;/i&gt; green fluorescent protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="142-147" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="7" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3779-3827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3841-3932" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3062-3166" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="902-1023" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3193-3648" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-2030" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2992" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2201-2992" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2993-3061" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>43cca133-d86c-4ee8-8238-6926f7159c2a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:52:57">2019.8.6</Created>
<LastModified UTC="8:18:41">2025.12.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="147..860" colors="green"/></TopStrand><BottomStrand><ColorRange range="147..860" colors="green"/></BottomStrand></StrandColors>  ¤      Ô           ld'ÄîœÈäÅxW…xW…xW…xW…xW‘¸	™`S Úðž(%O}�G=UÙQPü3Úæó�Fó�^6PæFsø+à]hê�Ý›�Ûzs„ž±X¼¬(4S˜W€ágVÝË&4…^îµ®¦<"€Ì¦¦Á(ÒŒüÅÇje…ÈZ;]&jÍ>|‚+*õT�Uj…ÈÕU;õ¶õU¦›`k•piô­,bw+-Û¶çïÂ©ag¢=úÆ@uhÞa{îç‚ãåB,î>Ù�Œ„k³cÑæªm[kÁž1ªR(¯kÛpªŠžw°f¡˜�«Ë¥‰‘¨QDâ®O­�FµHÎ8#×ù®úë=TÞá¡ÕœjnZ�Åhë‚ïû¹AK S
=D+Û�BmA˜Þçm9®wñ e|ÂÂT¢½ª¡LP¢àgÀUÎ)mQ^"!¾a¦/
bW[ÁL}sèhuv¸Ù:xqw®¶Ê>»†”�PæÐƒ¿8Z½º;××n±Í³BëñÆð…ëóÏ(¢¨pTq‡TFÈrÏKR]|ÅT£0A(ŠˆR|T.Ÿ^Ç5[DFûeCÔP0Tlò%®D°<<ëGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*©SRUû�X‚U±”Ëþ„O­KNúð€ÌaæË<Þ˜xºêm�{$ðÓ;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u¨ö³ÚÐã5â€P1°ŒºÎiÏOÔçØe%%šPœ' L<ÖU›©ªÚvâ"‚ªˆ…±’yvs»ÛUÕq]WW“U�âÿHÔ�ô]«.  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  »<?xml version="1.0"?><Features nextValidID="9"><Feature recentID="8" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="142-151" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="7" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4718-4766" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4780-4871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4001-4105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1841-1962" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4132-4587" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2381-2969" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3931" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3140-3931" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="3932-4000" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="luciferase" directionality="1" translationMW="60645.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="148-1800" color="#ffcc00" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc+&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;enhanced&amp;nbsp;&lt;i&gt;luc+&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>43cca133-d86c-4ee8-8238-6926f7159c2a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:52:57">2019.8.6</Created>
<LastModified UTC="8:18:4">2025.12.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	ý7zXZ  æÖ´F !   t/å£à:ÐÄ] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÛ¿¦2	#·bw…²Es<`ÄåÒ(f~lk›+ÚK'ÈÅ¯Ú[’Ùé,§-~‹Á|Apë™œÅ9‡å;E]ðr‰„°REôFzµÎ­6ê£¢Î&Ç3O•)Ýc4Èh�ä#bn=Ä}�Â9¤÷¢¿~:÷â£ëƒ¤"åh�  Âˆ%Ãk,’¤nGHçðî‰8ËTT´Åz¨¸²�H…×ÅQÍNfÃH,Û;Ä™»†ÞJî0-ÎBù¼K2ÕËêÔ‰¶©A:j×ð)à»÷	YÅ¤¢[hÿuèYX	¯�ôB˜8~TÀ¦CVö&àBéV]ÙRÆî·«0FìÑ×mq|–nÐŸ~¼­.z0”ç› f’’¡‚ãâJæ�ýµN-7“£˜ºù¢¡cR
-j±v�-=óùk1=7Ï¹ªÆ4ð4jñyF~÷šÚ=�'Î’“\ÖÿT†EÉ?c�+œÿ¤ýé·‚L!6a<Þ¬áíþ^MÛc)#É]S«µK½£XãnÈÒŠÝV„éÿ¹mw€¸	«ÑùË¡Ç@´>ôùªüè?" ã:…ýÐãž;.Ô™r—CÖRâŸ‹ ³·²:³<@îÉF¦öŠI›>?ìˆ&°9iˆ"ë‰I©~9Ö[º/Ñpá¦¼´»o§º+`X½ï1)¬±j¯{µ¥í™vX¤Í¸Šjà Å™H%0í£ ü:‘°z%jæŠájòB>‰G¢«,Ä«•±£z5]¢1ßmÝÏY»ü�.lX>‚-|Ë¦ÈdÖç'mÕ..Ô‚�«$ôÔ'ót1â%©©c-êV†’ƒl³qÙTlËž·­Äøx6±9 FI¨É¨‹€+ÇÊÍ…rWû°ŸïBŸœìÜ^Y²âPÇÇiÜüY(—s_îè¤÷¦a<:Y”ô–»ú­-:ÐN]ËuŽã.¹–BPÚý sŸÂ¦À#o}Tß†#ü¨Žµ:ž4„v¹)ÎÏ w ú t1õ;Ê'�ž±ž›ýJò¦™çUíNb“ˆèzàˆ�šLWäªúN-'4!OŸ‡ÕÙ(Ÿž8nÃ~Þ¥u~Û3•#§³“ÊÀ éÿG
¿›&3^ Ú×wÕJ^»Ú­�\LÕU)zƒ”Ø9pMÄQ¡,vJ½œ~!%«É²?ÒŽù‚�†	Á½—cÚ;°
ö¤gkd<æR�Âe…ø´'3¨üà:}9]ÿ@Uò�žØt\)!-�eaõ½…&f¼C}1Ý»ÅÃ	þjö.´C‡gjÒbÚ*Æoå‹$+¡wù|SY3Ct•üñé¹þ‹ÙE<U¤mÏrZ/™è5Ó¬�ÀN
K)ÔeT\Zž_}ù„÷&¯TìÙŠP¬¤‚«%F­*òUŒö×�%µ¶49 �ðp´ˆefk¦Žb›Wâx�ô8T4(ˆ&E,YeÎö^u$J(%„b~=Kï\F¾»sVË�ŒìâÃùÃ*V$ÂŸM›eÈ°Íx8</�Œrà…¨ìZ-zÖÈY^Žômõì³(%üµHÞÓåÝÍå¨³ò±ˆiŠ¡ÒÆì&H”�p/øSV™ÌÑ9 “l¥ºkÇ€nXks¸z.yã@zòtôDÆn†Že•Å…gÐ#ÙÎ
¸e7‰’O€ZÊ~æ'Š3¡ ì9ÆG2›©�âÇaVPº>(g�X%L#&Û
ãFzSå“~l±_î7oŽ•:Á×ñ³Ùæ'�Ñlw¨¹Äw�Ýîé†Ã”‘#œôv'€ L…Ñ?ëŒä¥Q0ÖræÂ•s‹@Ö×ØÔ‹…²®çzé<)šÅ ví±y¬\
¨¡†Õ‹•Ê7•,ä}¼Ý< ?¾ˆê¢„…ÖË9·Ô\“D¸ë��*ÐÐj–Žëgí%©;‘qì@·æÞ–|š%ÄÂÞý&ŸOÙï$ˆh¢½«kæ¯„þú±�°vd‚Xü)õéDøNÇº+±ný1ŠŠ·;Üå©ðEŽý=ÕÏ¢A”8FÀ¢ÿÊšFÙl*
ƒ½
ÿ!\—NŠ9CYÌØþ8J²Þyµ¾NÓÚT¶#cöèU¨íÁåÄÏPxƒ²ÚV(NíˆïÒÞYµðM%�S#¬ÃWl¥l9«Œ£ÿ™8æ^(ÖÿoûuÍe�(ÿá.ù¾G´Ü¦eÜ�ÔE…Ñ^ é» !÷áÄÑ$_åô tŽš4H<ñäC(ì��Ó‰}V;XIGrcÉ²0Þãr�¾…Q~1ÁïW%•’È¾c–yê�ëI
 ëˆ!ó9³Çá�‰¹oÿ{¸‘€7<ûx#§žá¨¾ÐJuV<¶CšÍ¢ÀÃxƒƒÙ2 ?FŒç™¥¹`6Q¤8ëü Å
bÖEJµ�êoP™0¦+ZîÞà°*<bƒfu�ÄÌ®¥*Yy ¬O‡�Ê.ê¦ÚP�a¬+ûdÅd£ˆ¡ÜnÔIÛsLã¹·{(/âo²„ý‡gSo7¼µé8LS�QAÉ^w
–R·fvG“}À>gð¬²F'µW%ÛÊ„ä÷èßx¹õ¿ðWÊ¼�T3àÖ&ïWwEÆÚG	ÒdË÷rÂ›ro%ÐïLl¾]}Æ©ù°ÒÜ¿ê5±kÔj0O„ÂÉM7¡òg¥>Y²Ù™¬q
Ý�ª©±·§¾mrn“h¨êÛ¯aö™1[t—¡(¦×x¡`½·ëcm`âJž§6OÆMã9Ñr—¢÷±û»UÿP|Mm™}ƒ¦Ô&RTe{N >¥á f6,ñšD¤ô%ºX^tö^wt	´I(ª€n£®%£Ê
¬zÑm¥w"½…mêèC¡)�˜„,�Ñ‰ƒÂò¡ÙûpMê €�$üç>  à˜jg—/ àÑu  *CD±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="0" name="ISRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="34-93" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ISRE-TA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14-130" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="GFP" directionality="1" translationMW="26797.80" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="148-861" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Aequoria victoria&lt;/i&gt; green fluorescent protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"/></Q></Feature><Feature recentID="3" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="142-147" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="4" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3778-3826" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3840-3931" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3061-3165" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="901-1022" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3192-3647" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1441-2029" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2991" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="2200-2991" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2992-3060" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>43cca133-d86c-4ee8-8238-6926f7159c2a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pISRE-TA-GFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="8:52:57">2019.8.6</Created>
<LastModified UTC="4:11:1">2026.3.31</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="13..129" colors="black"/><ColorRange range="147..860" colors="green"/></TopStrand><BottomStrand><ColorRange range="13..129" colors="black"/><ColorRange range="147..860" colors="green"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
